The pulsed electric field ablation instrument is composed of pulse ablation host, foot switch, operation screen, body surface and cardiac conductive line, etc. The host is composed of central control system, real-time ECG signal detection module and pulse generation unit. When in use, the host outputs pulsed electrical signals, which act on cardiomyocytes through the combined catheter to produce irreversible perforation effect, so as to realize the treatment of atrial fibrillation.